Journal: Experimental gerontology

Article Title: Sustaining neuromuscular activation after total knee arthroplasty preserves skeletal muscle fiber size, contractility, and innervation in older adults

doi: 10.1016/j.exger.2025.112831

Figure Lengend Snippet: Effect of NMES on the response to total knee arthroplasty (TKA) of skeletal muscle expression of genes activated upon skeletal muscle denervation via RNA fluorescent in situ hybridization (RNA-FISH), including the acetylcholine receptor sub-unit α1 ( CHRNA1 ) and myogenin ( MYOG ). Representative images for CHRNA1 (A) and MYOG (C) are shown using antibodies for laminin (green), fluorescently labeled in situ hybridization probes (red) and DAPI (blue), along with mean myonuclear intensity data for each analyte (B and D, respectively). Scale bar = 100 μm. Data represent mean and SE for n = 8 controls and n = 7 NMES patients. Data points represent individual values for volunteers for baseline and 5-week post-surgery evaluations. P value notations indicate time (T) and group X time (GxT) effects (atop brackets) or within group comparisons over time (over lines). * P < 0.05, ** P < 0.01, *** P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The next day, slides were incubated in goat anti-rabbit AF488 (#A-11034, ThermoFisher) and co-stained with DAPI (#D3571, ThermoFisher) prior to mounting with fluorescent mounting media (#H-1000, Vector).

Techniques: Expressing, In Situ Hybridization, Labeling